Anti Deep Ze 0.1
Species GlossaryAt=A. ThalianaSyHa=Golden Syrian HamsterAll=All SpeciesHa=HamsterAn=AnimalHu=HumanArHa=Armenian HamsterI=InsectAv=AvianLl=LlamaBb=BaboonMa=MammalBa=BacteriaMk=MonkeyBv=BovineMu=MouseCa=CanineMulti=Multi-speciesCe=C. ElegansNA=Non-species specificCh=ChickenOr=OrangutanChHa=Chinese HamsterPl=PlantCm=Cynomolgus MonkeyPm=PrimateDo=DonkeyPo=PorcineDr=DrosophiliaRb=RabbitEc=E. ColiRt=RatEq=EquineRM=Rhesus MacaqueFe=FelineSh=SheepFt=FerretVb=VertebrateFi=FishVi=VirusFu=FungiXp=XenopusGe=GerbilYe=YeastGP=Guinea PigZe=ZebrafishGt=Goat. This IRE1 pS724 antibody is useful for WB, ELISA, and IHC-P sections (PMID: 19264902). Use in IHC-Frozen reported in scientific literature (PMID 24823368).
Use in ICC reported in scientific literature (PMID 26762342). Use in immunoblotting reported in scientific literature (PMID: 24089213). Use In vitro assay reported in scientific literature (PMID: 24327956). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 25225294). Knockdown Validated reported in scientific literature (PMID: 31159306).Control.
Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and upregulates ER molecular chaperones in order to cope with ER stress. UPR is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha) (1). IRE1 alpha is correlated with X-box binding protein (XBP1) as a potent UPR transcriptional activator (2).Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress (3). UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER.IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress.
IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa.
When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.References1.Zheng, W., Xie, W., Yin, D., Luo, R., Liu, M., & Guo, F. ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling. Cell Commun Signal, 17(1), 42. Doi:10.1186/s12964-019-0353-32.Cho, Y. H., Jeong, S.
W., & Kwon, O. The IRE1alpha-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARgamma activity. Exp Mol Med, 50(8), 102.
Anti Deep Ze 0.1 100
Doi:10.1038/s12276-018-0131-03.Xia, Z., Wu, S., Wei, X., Liao, Y., Yi, P., Liu, Y. Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1alpha for cell survival. J Biol Chem, 294(37), 1. Doi:10.1074/jbc.RA119.008353 Limitations. Showing Publications 1 - 10 of 197.Publications using NB100-2323ApplicationsSpeciesWB, IHCMouseWBMouseWBMouseWBMouseWBHumanWBWB, Knockdown ValidatedMouseWBHumanWBRatWBMouseWBMouseWBMouseWBMouseWBMouseWBHumanHumanELISAMouseWBMouseWBWBMouseWBHumanICC/IF, WBMouseWBHumanMouseWBHumanWBHumanRatHumanWBHumanMouseMouseICC/IFIHC-PGoatHumanWBHumanIHCRatWBHumanHumanMouseWBMouseWBHumanWBMouseWBHumanWBRatWBMouseIHC, WBMouseHumanWBHumanWBMouseRatWBHumanWBMouseWBMouseWBHumanWBMouseWBMammalWBRatWBHumanWBRatWBHumanWBHumanHong M.
Regulation of Human Pancreatic B-cell Proliferation by Localization, Interaction, and Transcription of Endogenously Expressed Cell Cycle Molecules Thesis 2017 (Human)HumanWBMouseWBRatWBHumanWBHumanWBMouseWBMouseWBHumanWBMouse, RatWBRatWBMouseWBHumanWBHumanWBRatWBHumanWBMouseWBRatWBRatWBHumanWBHumanWBPrimateWBHumanWBHumanWBMouseICC/IF, WBMouseWBMouseWBRatImbernon Piedra M. Efectos centrales de MCH sobre el metabolismo periferico. Thesis Jun 1 2016 (WB, IHC)WB, IHCWBWBWBHumanWBMouseICC/IF, WBMouseIHCMouseWBWBMouseLu G, Ota A, Ren S et al. PPM1l encodes an inositol requiring-protein 1 (IRE1) specific phosphatase that regulates the functional outcome of the ER stress response. Molecular Metabolism. Summary ApplicationWestern BlotSample TestedWhole cell lysates: Hamster, Mouse, Human, MonkeySpeciesMouseLotAO-1Comments Comments6132 Pro cells (mouse fibrosarcoma) were treated with or without 10 mM DTT for 3 hr and total lysates were prepared.
The membrane blot was incubated with 5 ul of primary antibody (IRE1 alpha p Ser724) in 10 ml of blocker (2% BSA in TBS containing 0.05% Tween20) for 2hr at RT, followed by incubation with HRP-conjugated secondary antibody (anti-rabbit IgG H+L, from Pierce) for 1hr. The signal was visualized using chemilluminedcent substrate (Pico, from Pierce).
Summary ApplicationWestern BlotSample TestedMouse lens tissue, Sample Amount: 40ugSpeciesMouseLotIBlocking Blocking DetailsBlocking Buffer: 5% TBS+BSA, Blocking Time: overnight, Blocking Temp: 4 degrees CelsiusPrimary Anitbody Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: TBS+BSA, Incubation Time: 2 hours, Incubation Temp: 24 degrees CelsiusSecondary Antibody Secondary DescriptionSecondary Ab: ANTI-RABBIT (CALBIOCHEM), Secondary Ab Dilution Ratio: 1/20000Details Detection NotesDetection Method: Chemiluminescence, Exposure Time: 5 minutes, Specific Bands: 110 kDa. FAQs for IRE1 alpha Antibody (NB100-2323). (Showing 1 - 4 of 4 FAQs). Can you please tell me the immunogen sequence of this antibody?. The immunogen of NB100-2323 is a synthetic peptide from an amino acids sequence surrounding the phosphorylated serine 724 of the human IRE1 alpha protein (Swiss-Prot #O75460).
Unfortunately, I cannot provide any additional detail, as that is proprietary information. What is the size of the western blot band?. The weight observed in our in-house testing is typically 100-110kDa. Can this antibody detect non-phosphorylated IRE1a?.
NB100-2323 is phospho-specific. In all of our testing done with this antibody, we have only detected the phosphorylated IRE1a protein. There has not been any observed cross reactivity with the non-phosphorylated protein.
Anti Deep Ze 0.1 Video
I wish to use this antibody for cultured cardiomyocytes /Immunofluorescence. I am wondering if this antibody works for Immunocytochemistry/Immunofluorescence for cultured cells?
Specifically, we wish to compare IRE1 phosphorylation under stress condition to non-stress condition. Because the nature of IRE1 phosphorylation is part of stress response pathway. I am wondering if the immunofluorescence with your antibody is sensitive enough to distinguish p-IRE1 signal under stress condition to the endogenous stress (no stress inducer treatment) in the cell?. IRE1 p Ser724 antibody (NB100-2323) antibody has been validated in Immunocytochemistry/Immunofluorescence (ICC/IF) and we guarantee it 100% for the applications and species listed on our datasheet. As long as your cultured cardiomyocytes express the protein target that is phosphorylated at Ser 724 amino acid residue, our antibody will detect it. You may learn more about our guaranteee policy at https://www.novusbio.com/support/quality-assurance.html. Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U.Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg.The unfolded protein response (UPR) is a eukaryotic cell process that addresses ER stress.
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UPR is initiated by three ER-localized sensors: PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 alpha (.Inositol Requiring Enzyme 1 alpha (IRE1 alpha) is a transmembrane-RNase with an endoplasmic reticulum (ER) luminal sensor domain and cytosolic kinase and ribonuclease domains. IRE1 also plays a central role in the ER stress response (1). In a recent s.
What is AMH?. AMH, or anti-mullerian hormone is a substance produced by granulosa cells in ovarian follicles. It is first made in primary follicles that advance from the primordial follicle stage. At these stages follicles are microscopic and can not be seen by ultrasound. AMH production is highest in preantral and small antral stages (less than 4mm diameter) of development.
Production decreases and then stops as follicles grow. There is almost no AMH made in follicles over 8mm. Therefore, the levels are fairly constant and the AMH test can be done on any day of a woman's cycle. Research shows that the size of the pool of growing follicles is heavily influenced by the size of the pool of remaining primordial follicles (microscopic follicles in 'deep sleep'). Therefore, AMH blood levels are thought to reflect the size of the remaining egg supply - or 'ovarian reserve'.With increasing, the size of their pool of remaining microscopic follicles decreases.
Likewise, their blood AMH levels and the also decreases.Women with many small follicles, such as those with polycystic ovaries have high AMH hormone values and women that have few remaining follicles and those that are close to menopause have low anti-mullerian hormone levels. What is a normal AMH level?Interpretation of anti-mullerian hormone levels and chances for conceptionThere are some problems involved with interpretation of AMH hormone levels. Because the test has not been in routine use for many years, the levels considered to be 'normal' are not yet clarified and agreed on by all experts.The table below has AMH interpretation guidelines from the fertility literature and from our experience.
Do not get carried away with the cutoff values shown here. For example, the difference between a 0.9 and a 1.1 ng/ml test result puts a woman in a 'different box' in this table - but there is very little real difference in fertility potential. In reality, it is a continuum - and not something that categorizes well.
The ArcView 3.3 installer is a 16-bit installer and will not work on 64-bit operating systems. However, ArcView 3.3 can be manually installed onto a 64-bit operating. Create two new Keys, one called ArcView GIS Version 3.0 (note: NOT 3.3!),. Arcview gis 3.3 64 bit software. For 64bit Windows use target C:Program Files (x86)Common FilesESRI. This really does work: I have been using AV 3.3 in this mode for the last eight. Esri Support - Product Details: ArcView GIS 3.3 Product Life Cycle and Issues Addressed. Even thought it's deprecated, ArcView 3.3 is still a commercial GIS software. ArcView 3.x is a 32-bit app and has no problem running on a 32-bit or 64-bit.